Thermo Fisher Scientific IDH1 Polyclonal Antibody
IDH1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF에 사용 가능. 고순도 항원 친화 크로마토그래피로 정제되었으며, 인간 시료에 반응. 연구용으로 NADPH 생성 관련 대사 연구에 적합.
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Thermo Fisher Scientific IDH1 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–20 µg/mL |
| Immunocytochemistry (ICC/IF) | 5–20 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein Isocitrate Dehydrogenase 1, Soluble (amino acids 74–333 of target protein) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.67 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2903730 |
Target Information
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one utilizing NAD(+) and the other NADP(+) as electron acceptors. Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent (mitochondrial matrix) and two NADP(+)-dependent (one mitochondrial, one cytosolic).
The protein encoded by the IDH1 gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes, containing the PTS-1 peroxisomal targeting signal sequence. This enzyme plays a role in regenerating NADPH for intraperoxisomal reductions and in peroxisomal reactions consuming 2-oxoglutarate, including alpha-hydroxylation of phytanic acid.
The cytoplasmic enzyme contributes significantly to NADPH production.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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