Thermo Fisher Scientific TCR V beta 5.1/5.2 Monoclonal Antibody (MR9-4), PE, eBioscience

Thermo Fisher Scientific TCR V beta 5.1/5.2 Monoclonal Antibody (MR9-4), PE, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.25 µg/test

Product Specifications

Additional Formats

• Alexa Fluor 700
• APC
• eFluor 450
• FITC
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

This MR9-4 monoclonal antibody reacts with the mouse T cell receptor (TCR) V beta 5.1/5.2 chain. The MR9-4 antibody recognizes the V beta 5.1 and 5.2 chains on T cells from mouse strains with the b haplotype of the Tcrb gene, including C57BL/6, B10, and C58/J. V beta 5.1 and 5.2 are deleted in mouse strains that express MHC Class II I-E, including Balb/c, C3H, and DBA/2. Reports indicate that V beta 5+ T cells recognize the OVA peptide.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤ 0.25 µg per test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best performance.
Excitation: 488–561 nm
Emission: 578 nm
Laser: Blue, Green, Yellow-Green

Target Information

T cell receptors (TCR) discriminate foreign from self-peptides presented by MHC class II molecules, critical for adaptive immunity. Recognition of self-peptides is linked to autoimmune diseases; mutant self-peptides are associated with tumors. TCR engagement by bacterial superantigens causes toxic shock syndrome. Autoantibodies to V beta segments have been isolated from patients with RA and SLE, blocking TH1-mediated inflammation. Most human T cells express TCR alpha-beta with CD4 or CD8 (SP), while a subset lacks both (DN), which increases in autoimmune and immunodeficiency disorders. Gamma-delta T cells, mainly epithelial, show limited diversity and distinct antigen recognition, with known antitumor and immunoregulatory roles.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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