Thermo Fisher Scientific TCR V beta 5.1/5.2 Monoclonal Antibody (MR9-4), PE, eBioscience
마우스 TCR V beta 5.1/5.2 체인을 인식하는 MR9-4 단클론 항체로, PE 형광체가 결합되어 있습니다. Flow Cytometry 분석에 최적화되어 있으며, 0.25 µg/test로 사용 가능합니다. PBS 완충액에 보관하며 4°C, 암소에서 저장해야 합니다.
- 카탈로그번호
- 12-5796-82
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific TCR V beta 5.1/5.2 Monoclonal Antibody (MR9-4), PE, eBioscience
Thermo Fisher Scientific TCR V beta 5.1/5.2 Monoclonal Antibody (MR9-4), PE, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.25 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MR9-4 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2744702 |
Additional Formats
- Alexa Fluor 700
- APC
- eFluor 450
- FITC
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
This MR9-4 monoclonal antibody reacts with the mouse T cell receptor (TCR) V beta 5.1/5.2 chain. The MR9-4 antibody recognizes the V beta 5.1 and 5.2 chains on T cells from mouse strains with the b haplotype of the Tcrb gene, including C57BL/6, B10, and C58/J. V beta 5.1 and 5.2 are deleted in mouse strains that express MHC Class II I-E, including Balb/c, C3H, and DBA/2. Reports indicate that V beta 5+ T cells recognize the OVA peptide.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤ 0.25 µg per test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best performance.
Excitation: 488–561 nm
Emission: 578 nm
Laser: Blue, Green, Yellow-Green
Target Information
T cell receptors (TCR) discriminate foreign from self-peptides presented by MHC class II molecules, critical for adaptive immunity. Recognition of self-peptides is linked to autoimmune diseases; mutant self-peptides are associated with tumors. TCR engagement by bacterial superantigens causes toxic shock syndrome. Autoantibodies to V beta segments have been isolated from patients with RA and SLE, blocking TH1-mediated inflammation. Most human T cells express TCR alpha-beta with CD4 or CD8 (SP), while a subset lacks both (DN), which increases in autoimmune and immunodeficiency disorders. Gamma-delta T cells, mainly epithelial, show limited diversity and distinct antigen recognition, with known antitumor and immunoregulatory roles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.