Thermo Fisher Scientific NOTCH1 Monoclonal Antibody (22E5), PE, eBioscience

Thermo Fisher Scientific NOTCH1 Monoclonal Antibody (22E5), PE, eBioscience™

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test
Publications: -

Product Specifications

Additional Formats:

• APC
• Request custom conjugation

Product Specific Information

This 22E5 monoclonal antibody reacts with the extracellular domain of mouse Notch1, one of four members of the Notch family of receptors. Notch receptors are 300-kDa single-pass transmembrane proteins. The extracellular domain contains numerous epidermal growth factor-like repeats for ligand binding, while the intracellular domain is involved in cell signaling.

Upon binding its membrane-bound ligand (Delta or Jagged), the Notch receptor undergoes proteolytic cleavage by ADAM-family metalloproteases and gamma-secretase. The released Notch intracellular domain (NICD) translocates into the nucleus, binds RBP-J, recruits co-activators, and activates transcription.

Notch1 is expressed on thymocytes, bone marrow hematopoietic stem cells, T and NK cells, and at lower levels on B cells and monocytes. Certain T-cell acute lymphoblastic leukemia (T-ALL) subsets exhibit Notch1 chromosomal translocation with TCRβ, resulting in constitutively active Notch1. This receptor is involved in T cell lineage commitment, thymocyte development, and Th2 differentiation.

Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of mouse thymocytes at ≤0.5 µg/test (100 µL final volume).
Excitation: 488–561 nm
Emission: 578 nm
Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The Notch gene family encodes transmembrane proteins with multiple cysteine-rich EGF-like repeats in the extracellular region. Four mammalian Notch genes (Notch1–4) are involved in differentiation of the nervous system and other structures. EGF-like proteins Delta and Serrate act as ligands for Notch1. Mature Notch proteins are heterodimers formed by cleavage of Notch pre-proteins into an extracellular subunit (NEC) and a transmembrane subunit (Ntm).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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