Thermo Fisher Scientific NFS1 Monoclonal Antibody (OTI2H5), TrueMAB
인간 NFS1 단백질을 인식하는 단클론 항체로, Western blot 및 IHC(Paraffin) 실험에 적합합니다. 고순도 친화 크로마토그래피 정제 제품이며, 무보존제 PBS 버퍼에 동결건조 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific NFS1 Monoclonal Antibody (OTI2H5), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC(P)) | 1:150 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2H5 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–299 of human NFS1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional round of desalting (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
NFS1 (nitrogen fixation 1), also known as NIFS or IscS (cysteine desulfurase), belongs to the class V pyridoxal-phosphate-dependent aminotransferase family.
It localizes to the cytoplasm or mitochondrion depending on cytosolic pH.
Highest expression levels are found in heart and skeletal muscle, with lower levels in liver, brain, and pancreas.
NFS1 catalyzes the removal of sulfur from cysteine to form alanine, providing inorganic sulfur for iron-sulfur (Fe-S) clusters.
Fe-S clusters serve as essential cofactors in electron transfer processes, enzymatic catalysis, and structural maintenance of DNA repair enzymes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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