Thermo Fisher Scientific IL-2 Polyclonal Antibody, PeproTech

Thermo Fisher Scientific IL-2 Polyclonal Antibody (PeproTech)

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFAQSII STLT.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IL-2. Anti-Human IL-2-specific antibody was purified by affinity chromatography employing an immobilized Human IL-2 matrix.

Sandwich ELISA:
To detect Human IL-2 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-2 (500-P22Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human IL-2.

Western Blot:
To detect Human IL-2 by Western Blot analysis, use at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant Human IL-2 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes, and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, representing an unusual regulatory mode for precise expression control. Disruption of an IL-2-like gene in mice leads to an ulcerative colitis-like disease, suggesting an essential role in immune response to antigenic stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지

(이미지 파일명: 500-P22-100UG_IL-2_P60568-1_Rabbit.svg, 500-P22-100UG_IL-2_P60568-1_Rabbit_PDP.jpeg)