Thermo Fisher Scientific IL-2 Polyclonal Antibody, PeproTech
Thermo Fisher Scientific의 IL-2 Polyclonal Antibody는 인간 IL-2를 특이적으로 인식하는 토끼 유래 항체입니다. Western blot, ELISA, Neutralization 등 다양한 어세이에 사용 가능하며, 항원 친화 크로마토그래피로 정제되어 높은 특이성과 감도를 제공합니다. 연구용으로만 사용됩니다.
- 카탈로그번호
- 500-P22-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-2 Polyclonal Antibody, PeproTech
Thermo Fisher Scientific IL-2 Polyclonal Antibody (PeproTech)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | – |
| ELISA | 0.5–2.0 µg/mL | – |
| Neutralization (Neu) | – | 2 publications |
| In vitro Assay (IV) | – | 1 publication |
| Miscellaneous PubMed (Misc) | – | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human IL-2 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929879 |
Product Specific Information
AA Sequence of recombinant protein:
MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFAQSII STLT.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IL-2. Anti-Human IL-2-specific antibody was purified by affinity chromatography employing an immobilized Human IL-2 matrix.
Sandwich ELISA:
To detect Human IL-2 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-2 (500-P22Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human IL-2.
Western Blot:
To detect Human IL-2 by Western Blot analysis, use at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant Human IL-2 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Target Information
Interleukin 2 (IL-2) is an immuno-modulatory cytokine important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes, and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, representing an unusual regulatory mode for precise expression control. Disruption of an IL-2-like gene in mice leads to an ulcerative colitis-like disease, suggesting an essential role in immune response to antigenic stimuli.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일명: 500-P22-100UG_IL-2_P60568-1_Rabbit.svg, 500-P22-100UG_IL-2_P60568-1_Rabbit_PDP.jpeg)
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