Thermo Fisher Scientific IL-22 Monoclonal Antibody (22URTI), Brilliant Ultra Violet 395, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular Staining)
• Tested Dilution: 5 µL (0.25 µg)/test

Product Specifications

Available Formats

• Brilliant UV 563
• Brilliant UV 661
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 605
• eFluor 450
• eFluor 660
• FITC
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The 22URTI monoclonal antibody reacts with human interleukin (IL)-22, a 20 kDa cytokine of the IL-10 family secreted primarily by Th17 and NK cells. IL-22 expression is induced mainly by IL-23 and is associated with more differentiated Th17 cells. Its receptor is a heterodimer of IL-10R2 and IL-22R1.

Applications Reported:
For intracellular staining followed by flow cytometric analysis.

Applications Tested:
Pre-diluted and tested by intracellular staining of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00).
Recommended usage: 5 µL (0.25 µg) per test (100 µL final volume; 10⁵–10⁸ cells/test).

Fluorochrome Information:
Brilliant Ultra Violet™ 395 emits at 395 nm and is suitable for cytometers equipped with a 355 nm UV laser. Ensure instrument compatibility.

Buffer Recommendation:
When using multiple Brilliant Ultra Violet™ or polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product #SB-4401-42) or Brilliant Stain Buffer™ (Product #00-4409-75) to minimize non-specific interactions.

Excitation: 350 nm
Emission: 395 nm
Laser: Ultraviolet

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company and used under license. Powered by Sirigen™.

Target Information

IL-22 (also known as IL-10-related T-cell-derived inducible factor) is an alpha-helical cytokine in the IFN–IL-10 family, including IL-19, IL-20, IL-24, IL-26, IL-28, IL-29, and type I/II interferons. It is produced mainly by activated T cells and NK cells.
IL-22 signals through a receptor complex of IL-22R1 and IL-10R2, activating the JAK-STAT pathway. A soluble receptor, IL-22BP, can antagonize IL-22 signaling.

IL-22 primarily acts on non-immune cells (skin, digestive, respiratory, hepatic tissues) to regulate innate immunity, wound healing, and antimicrobial responses. It is co-expressed with IL-17A/F by Th17 cells and plays a key role in inflammation and host defense.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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