Thermo Fisher Scientific GOT1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:10-1:100

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 244-413 of human GOT1 (NP_002070.1). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GOT1,uniProtId:P17174-1,ncbiNodeId:9606,antigenRange:244-413,antigenLength:413,antigenImageFileName:PA5-120951_GOT1_P17174-1_Rabbit.svg,antigenImageFileNamePDP:PA5-120951_GOT1_P17174-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.55 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914523

Product Specific Information

Positive test controls include: HepG2, U-251MG, Mouse brain, Mouse liver, Mouse heart, Rat brain, Rat liver, Rat kidney. The target is usually found in the following locations: Cytoplasm.

Immunogen sequence: FVSEGFEFFC AQSFSKNFGL YNERVGNLTV VGKEPESILQ VLSQMEKIVR ITWSNPPAQG ARIVASTLSN PELFEEWTGN VKTMADRILT MRSELRARLE ALKTPGTWNH ITDQIGMFSF TGLNPKQVEY LVNEKHIYLL PSGRINVSGL TTKNLDYVAT SIHEAVTKIQ

Target Information

GOT1 is a glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.