Zenogen Pharma CELLBANKER® 2
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혈청 무첨가 세포 동결보존용 배지로 다양한 포유류 세포에 적용 가능. -80℃에서 직접 냉동 가능하며 고세포 생존율 유지. 추가 준비 불필요한 완전 조성으로 안전하고 효율적인 동결보존 제공. 20mL 및 100mL 포장.
브랜드: Zenogen Pharma
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For inquiries including COA (Certificate of Analysis), please contact:
zenogen_pharma-post@zenoaq.jp
Product Information
Description
CELLBANKER® 2 is a serum-free cell cryopreservation medium with a unique complete formulation for a broad spectrum of mammalian cell cultures.
The formulation enables stable cryopreservation and high viability after freeze-thaw procedures, minimizing contamination risks from serum components.
Optimum for serum-free cultured cells and peptide/protein expressing cells cryopreservation.
Advantages
- Safe: High cell viability, no risk of serum-derived pathogen contamination, batch-to-batch stability
- Save: Complete formulation, no further preparation, direct freezing at -80℃, no programmable freezer required
Package
| Volume | Description |
|---|---|
| 100 mL | Cryopreservative Medium (Serum free) |
| 20 mL | Cryopreservative Medium (Serum free) |
Both bottles are labeled “FOR RESEARCH USE ONLY” and manufactured by:
NIPPON ZENYAKU KOGYO CO., LTD.
1-1 Tairanoue Sasagawa, Asaka-machi, Koriyama, Fukushima 963-0196 Japan
Application
| Cell type | Description |
|---|---|
| 293 | Sheared human Ad5 DNA-transformed cell line |
| 293T | Human cell line expressing SV40 large T antigen |
| 32D | Murine myeloid cell |
| 3LL | Murine Lewis lung cancer cell |
| A10 | Thoracic aortic smooth muscle of an embryonic rat |
| A2781 | Glioblastoma cell |
| Ac2F | Rat liver cell |
| AtT20 | Murine anterior pituitary cell |
| Ba/F3 | Murine Pro-B cell |
| BHK-21 | Baby Hamster renal fibroblast cell |
| C2C12 | Mouse myoblast cell |
| C3H10T1/2 | Murine embryonic fibroblast cell |
| Caco-2 | Human colonic adenocarcinoma cell |
| CHL/1U | Neonatal Chinese Hamster Lung cell |
| CHO | Chinese Hamster Ovary cell |
| CHO-K1 | Chinese Hamster Ovary cell |
| Colo203 | Human colonic adenocarcinoma cell |
| COS1 | African green monkey kidney cell |
| COS7 | African green monkey kidney cell |
| CTLL-2 | Murine T-cell |
| DLP-1 | Human colonic adenocarcinoma cell |
| DT40 | Chicken B lymphocyte cell |
| DU145 | Human prostate carcinoma cell |
| EBV transformed B cell | |
| EJ-1 | Human bladder carcinoma |
| ELM-D | Murine erythroblastic leukemia cell |
| HeLa | Human uterine cervical carcinoma cell |
| Hep3B | Human hepatoma cell |
| Hepal-6 | Murine hepatoma cell |
| Hepatocyte | |
| HepG2 | Hepatocellular carcinoma cell |
| HL-60 | Human promyelocytic leukemia cell |
| HT-2 | Murine cell |
| Huh-7 | Human hepatocarcinoma cell |
| Jurkat | Human T-cell line |
| K-562 | Human chronic myelogenous leukemia cell |
| Kato-III | Human stomach carcinoma |
| Keratinocyte | |
| KM12-LX | Human chronic myelogenous leukemia cell |
| L5178Y | Murine lymphoma cell |
| L929 | Murine fibroblast cell |
| LM | Murine fibroblast cell |
| LNCap | Human prostate adenocarcinoma cell |
| Lymphocyte | |
| MCF-7 | Human metastatic mammary carcinoma cell |
| MDCK | Madin-Dar canine kidney cell |
| Microvascular Endothelial Cell | |
| Molt-4 | Human T-cell |
| NB-1/GOTO | Human neuroblastoma |
| NCI-H441 | Human lung adenocarcinoma epithelial cell |
| NIH3T3 | Mouse embryonic fibroblast cell |
| P388 | Murine leukemia cell |
| P3/x63-Ag8.U1 | Murine myeloma cell |
| PC12 | Rat pheochromocytoma cell |
| Periodontal ligament fibroblast cell | |
| Periodontal ligament membrane fibroblast cell | |
| Raji | Human B-cell line |
| RAW264.7 | Murine macrophage cell |
| sf9 | Insect cell |
| SK-N-MC | Neuroepithelioma cell |
| SN12C | Renal carcinoma cell (Subclone: PM6, Clone8, MM3) |
| Stem cell | |
| Stomach carcinoma cell | |
| THle3 | Human liver cell |
| T24 | Human bladder carcinoma |
| Vero | African green monkey kidney epithelial cell |
| WEHI3B | Murine myeloid leukemia |
| WiDr | Human colon adenocarcinoma cell |
Typical Experimental Results
| Cells | Preservation Period (year) | Viability (%) at -80℃ |
|---|---|---|
| Mouse | ||
| Hybridoma | 5 | 90 |
| Myeloma | 5 | 90 |
| L929 | 5 | 90 |
| FM3A | 5 | 90 |
| BALB/3T3 | 5 | 90 |
| Rat | ||
| RLC-16 | 5 | 90 |
| NKR | 3 | 90 |
| Hamster | ||
| CHO | 3 | 90 |
| V79 | 3 | 90 |
| Monkey | ||
| COS-1 | 3 | 90 |
| Vero | 5 | 90 |
| Human | ||
| EBV transformed cell | 5 | 90 |
| Fibroblast | 5 | 90 |
| Melanoma | 5 | 90 |
| SK-007 | 5 | 90 |
| Jurkat | 5 | 90 |
| K562 | 5 | 90 |
Protocol
Freezing
For optimum results, cells should be in log phase of growth.
Standard freezing protocols may be applied.
- Ensure cell culture is healthy and contamination-free.
- Perform a cell count to determine viability.
- Centrifuge (3–5 min at 1,000–2,000 rpm, 4℃) and remove supernatant.
- Suspend cells in CELLBANKER® medium (1 mL for 5×10⁵–5×10⁶ cells).
- Dispense 1 mL aliquots into labeled cryovials.
- Store directly at -80℃. Optionally transfer to liquid nitrogen after 24 h.
IMPORTANT: Optimum protocol may vary by cell type.
Procedure for Use (from image):
- Culture
- Centrifuge
- Remove Supernatant
- Suspend with CELLBANKER
- Aliquot
- -80°C (directly)
- Optional
Thawing
- Quickly thaw frozen cells in a 37℃ shaking water bath.
- Dilute each 1 mL of cells with 10 mL of complete medium.
- Centrifuge (3–5 min at 1,000–2,000 rpm, 4℃) and remove supernatant.
- Resuspend cells in complete medium and plate in a culture flask.
- Continue culture as standard.
Storage and Stability
Store at 2–8℃ or below -20℃.
Stable for 3 years after manufacture under unopened condition at 2–8℃.
SDS
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