Thermo Fisher Scientific nNOS Polyclonal Antibody
Rabbit polyclonal antibody against rat nNOS. Validated for Western blot and IHC. Detects ~155 kDa nNOS protein, no cross-reactivity with iNOS or eNOS. Supplied as liquid, 1 mg/mL, stored at -20°C. For research use only.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:100 | View 6 publications |
| Immunohistochemistry (IHC) | - | View 1 publication |
Product Specifications
| Category | Specification |
|---|---|
| Published species | Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues I(237) Q V D R D L D G K S H K A(250) of rat nNOS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_325775 |
Product Specific Information
PA1-038 detects recombinant rat brain nitric oxide synthase (nNOS) overexpressed in E. coli.
This antibody does not detect iNOS or eNOS.
- Successfully used in Western blot procedures
- Detects an ~155 kDa protein representing recombinant rat nNOS protein overexpressed in E. coli
- Immunogen: synthetic peptide corresponding to residues I(237) Q V D R D L D G K S H K A(250) of rat nNOS
Target Information
Nitric oxide synthase (NOS) activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH, and O₂) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme).
Several distinct NOS isoforms have been described and represent the products of three distinct genes:
- NOS1 (ncNOS): neuronal form, Ca²⁺/CaM-dependent
- NOS3 (ecNOS): endothelial form, Ca²⁺/CaM-dependent
- iNOS: inducible form
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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