Thermo Fisher Scientific nNOS Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100

View 6 publications 6 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Product Specifications

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues I(237) Q V D R D L D G K S H K A(250) of rat nNOS. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:nNOS,uniProtId:P29476-1,ncbiNodeId:10114,antigenRange:237-250,antigenLength:1429,antigenImageFileName:PA1-038_nNOS_P29476-1_Rabbit.svg,antigenImageFileNamePDP:PA1-038_nNOS_P29476-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_325775

Product Specific Information

PA1-038 detects recombinant rat brain nitric oxide synthase (nNOS) overexpressed in E. coli. This antibody does not to detect iNOS or eNOS.

PA1-038 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~155 kDa protein representing recombinant rat nNOS protein overexpressed in E. coli.

The PA1-038 immunogen is a synthetic peptide corresponding to residues I(237) Q V D R D L D G K S H K A(250) of rat nNOS.

Target Information

NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.