Thermo Fisher Scientific AKAP3 Polyclonal Antibody

Applications

• Western Blot (WB): 1:500–1:1,000

Product Specifications

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity chromatography using an epitope-specific immunogen. The purity is >95% as determined by SDS-PAGE.

Target Information

The type II cAMP-dependent protein kinase (PKA) is a multifunctional kinase with a broad range of substrates. Specificity of PKA signaling is mediated by compartmentalization of the kinase to specific sites within the cell. To maintain this localization, the R subunit (RII) of PKA interacts with specific RII-anchoring proteins, designated A-kinase anchoring proteins (AKAPs).

AKAP3 (also known as AKAP110, FSP95, PRKA3, and SOB1) binds both PKA and PDE4A and functions as a scaffolding protein in spermatozoa to regulate local cAMP concentrations and modulate sperm functions. Expression of AKAP3 in normal tissues is restricted to the testis, where bicarbonate stimulates tyrosine phosphorylation of AKAP3, thereby increasing its recruitment of PKA.

AKAP3 also exhibits high expression in patients with epithelial ovarian cancer (EOC). It demonstrates tumor-restricted expression and appears to be associated with worse overall survival, making AKAP3 a potential target for antigen-specific immunotherapy in EOC.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.