Thermo Fisher Scientific CD357 (AITR/GITR) Monoclonal Antibody (DTA-1), APC, eBioscience

Applications

• Flow Cytometry (Flow): Tested dilution 0.06 µg/test
• View 7 publications

Product Specifications

Available Formats

• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The DTA-1 monoclonal antibody reacts with mouse GITR (Glucocorticoid-Induced TNFR family gene, TNFRSF18).
In naive mice, GITR is expressed predominantly by CD4⁺CD25⁺ regulatory T cells (Treg) and by CD25⁺CD4⁺CD8⁻ thymocytes.
Stimulation of GITR with DTA-1 antibody abrogates Treg-mediated suppression.
Although it does not deplete CD357-expressing cells in vivo, it has been shown to induce tumorogenesis.
Removal of GITR-expressing Treg cells or administration of DTA-1 resulted in organ-specific autoimmune disease.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤0.06 µg/test (100 µL final volume).
Cell number should be determined empirically (10⁵–10⁸ cells/test).
Titrate for optimal performance.

Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also known as GITR or AITR, is encoded by the TNFRSF18 gene (1p36.33).
It belongs to the TNF-receptor superfamily and shows increased expression upon T-cell activation.
It plays a key role in immunological self-tolerance maintained by CD25⁺CD4⁺ regulatory T cells.
Knockout studies in mice indicate its role in CD3-driven T-cell activation and programmed cell death.
Three alternatively spliced transcript variants encoding distinct isoforms have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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