Thermo Fisher Scientific TCR beta Monoclonal Antibody (H57-597), eBioscience

Thermo Fisher Scientific TCR beta Monoclonal Antibody (H57-597), eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugate Formats

• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 496
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 421
• eFluor 450
• eFluor 506
• FITC
• Functional Grade
• NovaFluor Blue 510
• NovaFluor Blue 530
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 725
• NovaFluor Blue 800
• NovaFluor UV 765
• NovaFluor Violet 690
• NovaFluor Violet 800
• NovaFluor Yellow 810
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780

Product Specific Information

Description:
The H57-597 monoclonal antibody reacts with the beta chain of mouse TCR. TCR beta is expressed by thymocytes in a developmentally regulated manner and by a majority of peripheral T cells. Crosslinking of the TCR complex with H57-597 induces activation and proliferation of T cells or apoptosis depending on assay conditions. H57-597 serves as a phenotypic marker for TCR beta-expressing T cells.

Applications Reported:

• Flow cytometric analysis
• Immunohistochemical staining
• In vitro functional studies
• In vivo depletion

For functional assays, use Functional Grade purified H57-597 (Product #16-0691).

Applications Tested:
Tested by flow cytometric analysis of mouse thymocytes and splenocytes. Recommended use ≤0.25 µg per test (100 µL final volume). Cell number may range from 10⁵ to 10⁸ cells/test. Antibody titration is recommended for optimal performance.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

T cell receptors (TCRs) are essential for recognizing foreign versus self-peptides presented by MHC class II molecules, enabling adaptive immune responses. TCR recognition of self-peptides has been linked to autoimmune diseases and tumors. Superantigen engagement of TCRs can cause toxic shock syndrome. Autoantibodies to V beta segments have been found in rheumatoid arthritis and lupus patients, potentially modulating TH1-mediated inflammation.

Most human T cells express TCR alpha-beta along with CD4 or CD8 (single positive). A subset of double-negative (DN) alpha-beta T cells increases in autoimmune and immunodeficiency disorders. Gamma-delta T cells, mainly in epithelial tissues, exhibit distinct antigen recognition and have shown antitumor and immunoregulatory roles.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.