Thermo Fisher Scientific NK1.1 Monoclonal Antibody (PK136), eBioscience
Mouse NK1.1 항원을 인식하는 PK136 단클론 항체로, NK 세포 및 일부 T 세포 분석에 적합. Flow cytometry, immunoprecipitation, 기능적 분석 등에 사용 가능. Unconjugated 형식으로 제공되며, 다양한 형광 및 기능적 등급 포맷 선택 가능.
- 카탈로그번호
- 14-5941-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific NK1.1 Monoclonal Antibody (PK136), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 1 |
| Immunohistochemistry (IHC) | - | 3 |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 1 |
| Immunocytochemistry (ICC/IF) | - | 1 |
| Flow Cytometry (Flow) | 0.5 µg/test | 130 |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| Neutralization (Neu) | - | 3 |
| Functional Assay (Functional) | - | 6 |
| Miscellaneous PubMed (Misc) | - | 3 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PK136 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467736 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Additional Formats Available
- Alexa Fluor 561
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395, 496, 615, 661, 805
- Brilliant Violet 421, 480, 605, 650, 711, 786
- eFluor 450
- FITC
- Functional Grade
- PE, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436, 600, 645, 702, 780
- Custom conjugation available upon request
Product Specific Information
The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in NK1.1 mouse strains including C57BL and NZB. Common laboratory mouse strains such as BALB/c, SJL, AKR, CBA, C3H, and A do not express NK1.1 antigen. For detection of NK cells in these strains, the monoclonal antibody DX5 should be used.
Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 reveals coexpression of both markers by most cells, as well as small populations of DX5⁺PK136⁻ and DX5⁻PK136⁺ cells.
Applications Reported: PK136 can be used for flow cytometric analysis, immunoprecipitation, depletion of NK cells, and in vitro functional studies.
For functional assays, use Functional Grade purified PK136.
Applications Tested: The PK136 antibody has been tested by flow cytometric analysis of C57Bl/6 mouse splenocytes and can be used at ≤0.5 µg per test (100 µL final volume). Cell number may vary (10⁵–10⁸ cells/test). Antibody titration is recommended for optimal performance.
Target Information
NK1.1 (KLRB1, NKR-P1A, CD161) refers to Natural Killer (NK) cells that mediate cytotoxicity and secrete cytokines upon immune stimulation.
The KLRB1 protein contains an extracellular domain with C-type lectin motifs, a transmembrane domain, and a cytoplasmic domain.
It is classified as a type II membrane protein with an external C-terminus.
NKR-P1A recognizes Lectin Like Transcript-1 (LLT1) as a functional ligand.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.