Thermo Fisher Scientific NK1.1 Monoclonal Antibody (PK136), eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• Alexa Fluor 561
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 615, 661, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450
• FITC
• Functional Grade
• PE, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 702, 780
• Custom conjugation available upon request

Product Specific Information

The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in NK1.1 mouse strains including C57BL and NZB. Common laboratory mouse strains such as BALB/c, SJL, AKR, CBA, C3H, and A do not express NK1.1 antigen. For detection of NK cells in these strains, the monoclonal antibody DX5 should be used.

Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 reveals coexpression of both markers by most cells, as well as small populations of DX5⁺PK136⁻ and DX5⁻PK136⁺ cells.

Applications Reported: PK136 can be used for flow cytometric analysis, immunoprecipitation, depletion of NK cells, and in vitro functional studies.
For functional assays, use Functional Grade purified PK136.

Applications Tested: The PK136 antibody has been tested by flow cytometric analysis of C57Bl/6 mouse splenocytes and can be used at ≤0.5 µg per test (100 µL final volume). Cell number may vary (10⁵–10⁸ cells/test). Antibody titration is recommended for optimal performance.

Target Information

NK1.1 (KLRB1, NKR-P1A, CD161) refers to Natural Killer (NK) cells that mediate cytotoxicity and secrete cytokines upon immune stimulation.
The KLRB1 protein contains an extracellular domain with C-type lectin motifs, a transmembrane domain, and a cytoplasmic domain.
It is classified as a type II membrane protein with an external C-terminus.
NKR-P1A recognizes Lectin Like Transcript-1 (LLT1) as a functional ligand.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.