Thermo Fisher Scientific VISTA Chimeric Recombinant Rabbit Monoclonal Antibody (13F3)
마우스 VISTA 단백질에 특이적인 키메릭 재조합 토끼 단클론 항체. Flow Cytometry, ELISA, Inhibition Assay 등 다양한 분석에 사용 가능. 고순도 Protein A 정제, 1 mg/mL 농도, 장기 보관 시 -20°C에서 안정적. 연구용으로만 사용.
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Thermo Fisher Scientific VISTA Chimeric Recombinant Rabbit Monoclonal Antibody (13F3)
Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | Assay-dependent |
| ELISA (ELISA) | 10,000 ng/mL |
| Inhibition Assays (IA) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG, lambda |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 13F3 |
| Immunogen | This antibody was raised by immunising Armenian hamsters with EL4 cells overexpressing VISTA-RFP, and VISTA-Ig fusion protein. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074679 |
Product Specific Information
Specificity: This antibody is specific for murine VISTA.
Target Information
VISTA (V-domain Ig containing suppressor of T-cell activation), also known as B7-H5 or PD-1H, is a product of the VSIR gene.
Although VISTA bears sequence homology to PD-L1, it has a distinct expression pattern. In both mouse and humans, VISTA is expressed predominantly on hematopoietic cells — most abundantly on myeloid cells and at lower levels on T cells, while being typically absent on B cells.
VISTA expressed by antigen-presenting cells directly suppresses proliferation and cytokine production of CD4 and CD8 T cells, making it a potential target for cancer immunotherapy.
Reported ligands of VISTA include CD28H, VSIG-3, and VSIG-8.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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