Thermo Fisher Scientific Estrogen Receptor alpha Monoclonal Antibody (33)
인간 ERα를 특이적으로 인식하는 마우스 단클론 항체로, Western blot, IHC, ICC 등 다양한 응용에 적합. ERβ와 교차반응 없음. 1 mg/mL 농도의 액상 제품이며 Protein G로 정제됨. -20°C 보관, 반복 냉동/해동 피함.
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Thermo Fisher Scientific Estrogen Receptor alpha Monoclonal Antibody (Clone 33)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 4 µg/mL | View 17 publications |
| Immunohistochemistry (IHC) | 1:20 - 1:100 | View 11 publications |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | View 7 publications |
| Flow Cytometry (Flow) | - | View 2 publications |
| Immunoprecipitation (IP) | - | View 2 publications |
| Gel Shift (GS) | - | View 3 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Non-human primate, Rat |
| Published Species | Avian, Bovine, Fish, Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 33 |
| Immunogen | Synthetic peptide corresponding to residues E(247)-G(261) of ER alpha DNA binding domain |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | Dulbecco’s PBS with 30% glycerol, 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_325422 |
Product Specific Information
MA1-310 detects both steroid-occupied and unoccupied forms of estrogen receptor (ER) alpha from human samples.
It does not cross-react with ER beta.
This antibody has been successfully used in Western blot, immunohistochemistry, immunoprecipitation, gel shift, flow cytometry, and immunocytochemistry.
By Western blot, it detects an approximately 66 kDa ER alpha protein from MCF-7 cell extract.
Can be used with blocking peptide PEP-013.
Target Information
Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors.
When bound to estrogen, ER acts as a ligand-activated transcription factor that dimerizes and binds to estrogen response elements (ERE) in DNA, regulating gene transcription through coactivator or corepressor recruitment.
There are two forms of ER, alpha and beta, encoded by ESR1 and ESR2 respectively.
At least four ER-alpha isoforms exist due to alternative RNA splicing:
- Isoform 1 (66 kDa)
- Isoform 2 (53 kDa)
- Isoform 3 (47 kDa)
- Isoform 4 (35 kDa)
ERs are expressed in various tissues and are essential for sexual development and reproductive function, as well as in bone and other tissues.
They are implicated in diseases such as breast cancer, endometrial cancer, and osteoporosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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