Thermo Fisher Scientific Estrogen Receptor alpha Monoclonal Antibody (33)

Applications

Tested Dilution

Publications

Western Blot (WB)

4 µg/mL

View 17 publications 17 publications

Immunohistochemistry (IHC)

1:20 - 1:100

View 11 publications 11 publications

Immunocytochemistry (ICC/IF)

10 µg/mL

View 7 publications 7 publications

Flow Cytometry (Flow)

-

View 2 publications 2 publications

Immunoprecipitation (IP)

-

View 2 publications 2 publications

Gel Shift (GS)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Non-human primate, Rat

Published species

Avian, Bovine, Fish, Human, Mouse, Non-human primate, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

33

Immunogen

Synthetic peptide corresponding to the residues E(247) V G M M K G G I R K D R R G(261) of the ER alpha DNA binding domain. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Estrogen Receptor alpha,uniProtId:P03372-1,ncbiNodeId:9606,antigenRange:247-261,antigenLength:595,antigenImageFileName:MA1-310_Estrogen_Receptor_alpha_P03372-1_House_mouse.svg,antigenImageFileNamePDP:MA1-310_Estrogen_Receptor_alpha_P03372-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

Dulbecco`s PBS with 30% glycerol, 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325422

Product Specific Information

MA1-310 detects both the steroid occupied and unoccupied forms of the estrogen receptor (ER) alpha from human samples. MA1-310 detects ER alpha and does not cross react with ER beta.

MA1-310 has been successfully used in Western blot, immunohistochemistry, immunoprecipitation, gel shift, flow cytometry, and immunocytochemical experiments. By Western blot, this antibody detects an ~66 kDa protein representing ER alpha from MCF-7 cell extract.

MA1-310 can be used with blocking peptide PEP-013.

Target Information

Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.