Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (ACK2), APC-eFluor 780, eBioscience
마우스 CD117(c-Kit) 인식 단일클론 항체로, 유세포분석(Flow Cytometry)에 최적화된 제품입니다. APC-eFluor 780 형광체로 결합되어 적색 레이저(633 nm)에서 검출 가능하며, 0.125 µg/test 농도로 사용됩니다. 4°C 암조건 보관, 블로킹 항체로 보고됨.
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Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (ACK2), APC-eFluor 780
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.125 µg/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Hamster, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), APC-eFluor™ 780, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ACK2 |
| Conjugate | APC-eFluor™ 780 |
| Excitation / Emission Max | 756 / 785 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1582227 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 700
- APC
- Functional Grade
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 702
- Request custom conjugation
Product Specific Information
The ACK2 monoclonal antibody reacts with mouse CD117 (c-Kit receptor), also known as Steel factor receptor or stem cell factor receptor. This 145 kDa tyrosine kinase receptor is expressed by hematopoietic progenitor cells, mast cells, and plays a role in cell signaling and activation. ACK2 is reported to be a blocking antibody.
Applications Reported:
For use in flow cytometric analysis.
Applications Tested:
Tested on mouse bone marrow cells at ≤0.125 µg/test (100 µL final volume). Recommended to titrate for optimal performance.
Fluorophore Information:
APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Ensure instrument compatibility.
Light Sensitivity:
Sensitive to photo-induced oxidation; protect vial and samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark.
Excitation / Emission: 633–647 nm / 780 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
KIT (c-KIT) is a proto-oncogene encoding a type 3 transmembrane receptor for mast cell growth factor (MGF or stem cell factor). It regulates growth and activation of hematopoietic and non-hematopoietic cells. Mutations in KIT are linked to gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism.
Activated c-Kit triggers multiple signaling pathways (PI3K, PLC-γ, JAK/STAT) essential for hematopoiesis, stem cell maintenance, and gametogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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