Thermo Fisher Scientific CD3 Monoclonal Antibody (OKT3), Alexa Fluor 488, eBioscience
인간 CD3 복합체의 epsilon 서브유닛을 인식하는 OKT3 단클론 항체입니다. Alexa Fluor 488으로 표지되어 있으며, 유세포분석(Flow Cytometry)에 최적화되어 있습니다. T세포 활성화 연구에 적합하며, 4°C 암소 보관이 권장됩니다.
- 카탈로그번호
- 53-0037-4x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD3 Monoclonal Antibody (OKT3), Alexa Fluor 488, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 3 |
| Immunohistochemistry (IHC) | - | 2 |
| Immunocytochemistry (ICC/IF) | - | 3 |
| Flow Cytometry (Flow) | 5 µL (1 µg)/test | 28 |
| Immunoprecipitation (IP) | - | 1 |
| Functional Assay | - | 1 |
| Immunomicroscopy (IM) | - | 1 |
| T-Cell Activation (TCA) | - | 9 |
| Miscellaneous (PubMed) | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Alexa Fluor™ 488 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OKT3 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1907371 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 563 / 615 / 661 / 737 / 805
- Brilliant Violet 421 / 605
- eFluor 450 / 506 / 660
- FITC
- Functional Grade
- NovaFluor Blue 510 / 585
- NovaFluor Yellow 590 / 660 / 730
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702 / 780
- Request custom conjugation available
Product Specific Information
The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. It exhibits potent immunosuppressive properties in vivo and has been used effectively in the treatment of renal, heart, and liver allograft rejection.
CD3 subunits (gamma, delta, epsilon) are essential for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes and all mature T cells. Crosslinking of TCR triggers intracellular signaling leading to T-cell activation and proliferation.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated for flow cytometry of normal human peripheral blood cells.
Use at 5 µL (1 µg) per test in a final volume of 100 µL (10⁵–10⁸ cells/test).
Excitation: 488 nm Emission: 519 nm Laser: Blue
Filtration: 0.2 µm post-manufacturing filtered
Target Information
The CD3 complex transduces antigen-recognition signals into T cells and regulates TCR surface expression. It includes subunits CD3 gamma, delta, epsilon, and zeta, encoded on human chromosome 11. These subunits have cytoplasmic tails that interact with signaling molecules via tyrosine-based motifs.
CD3 antigens are found on 68–82% of normal peripheral blood lymphocytes and 65–85% of thymocytes, but not on B or NK cells. Reduced T-cell percentages may occur in autoimmune diseases. Mutations in CD3 genes are associated with CD3 immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.