Thermo Fisher Scientific CD3 Monoclonal Antibody (17A2), Alexa Fluor 488, eBioscience
마우스 CD3 복합체를 인식하는 17A2 클론 단일클론 항체로 Alexa Fluor 488 형광 표지. Flow cytometry에 최적화되어 있으며, T 세포 활성화 및 증식 연구에 적합. 499/520 nm의 형광 특성, 4°C 암소 보관.
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Thermo Fisher Scientific CD3 Monoclonal Antibody (17A2), Alexa Fluor 488, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 1 publication |
| Immunohistochemistry (IHC) | - | 11 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 2 publications |
| Immunocytochemistry (ICC/IF) | - | 4 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | 55 publications |
| Functional Assay | - | 1 publication |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Alexa Fluor™ 488 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 17A2 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2848413 |
Available Formats
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395, 496, 737, 805
- Brilliant Violet 421, 480, 650
- eFluor 450, 506, 660
- FITC
- Functional Grade
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 600, 702, 780
- Custom conjugation available upon request
Product Specific Information
The 17A2 monoclonal antibody reacts with the mouse CD3 complex. CD3 subunits gamma, delta, and epsilon are required for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 17A2 to CD3 initiates intracellular signaling pathways leading to cellular activation and proliferation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Flow cytometric analysis of mouse splenocytes
- Recommended use ≤ 0.5 µg per test (100 µL final volume)
- Cell count range: 10⁵–10⁸ cells/test
- Antibody should be titrated for optimal performance
- Excitation: 488 nm
- Emission: 519 nm
- Laser: Blue Laser
Target Information
The CD3 subunit complex is essential for transducing antigen-recognition signals into the cytoplasm of T cells and regulating TCR expression. CD3 subunits (gamma, delta, epsilon, zeta) are members of the immunoglobulin superfamily encoded on chromosome 11. These subunits associate with cytoplasmic signal transduction molecules via tyrosine-based motifs. CD3 is expressed on 68–82% of peripheral blood lymphocytes and 65–85% of thymocytes, but not on B or NK cells. CD3 defects are associated with immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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