Thermo Fisher Scientific VDR Monoclonal Antibody (9A7)
VDR 단백질을 검출하는 Thermo Fisher Scientific의 Rat monoclonal antibody (Clone 9A7). Western blot, IHC, ICC, IP, ChIP 등 다양한 응용에 사용 가능. 인간, 생쥐, 닭 등 여러 종에 반응하며 비특이적 교차 반응 없음. Protein G 정제, PBS buffer, -20°C 보관.
- 판매단위
- pk
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Thermo Fisher Scientific VDR Monoclonal Antibody (9A7)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | 46 |
| Immunohistochemistry (IHC) | - | 28 |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 5 |
| Immunocytochemistry (ICC/IF) | 1:10–1:100 | 9 |
| Flow Cytometry (Flow) | - | 1 |
| Immunoprecipitation (IP) | Assay-dependent | 3 |
| ChIP assay (ChIP) | Assay-dependent | 3 |
| Gel Shift (GS) | Assay-dependent | 8 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chicken, Human, Mouse, Rat, and others |
| Published Species | Avian, Chicken, Dog, Hamster, Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rat / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9A7 |
| Immunogen | Partially purified chicken intestinal cytoplasmic 1,25-(OH)₂D₃ receptor protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_326366 |
Product Specific Information
MA1-710 detects vitamin D receptor (VDR) from human, rat, mouse, hamster, chicken, amphibian, and fish tissues. This antibody does not cross-react with estrogen or glucocorticoid receptors. It detects both occupied and unoccupied forms of the receptor.
MA1-710 has been successfully used in Western blot, IF, ICC, IP, ChIP, and gel shift procedures.
In Western blot, this antibody detects a 58–60 kDa protein representing VDR in avians, while mammalian VDRs appear as single bands within the 52–55 kDa range.
The immunogen is partially purified chicken intestinal cytoplasmic 1,25-(OH)₂D₃ receptor protein.
This antibody recognizes an epitope between amino acid residues 89–105 of human VDR, located just C-terminal to the DNA binding domain.
Target Information
Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to DNA sequences after activation by the appropriate hormone.
The 1,25-dihydroxy-vitamin D₃ receptor (VDR) belongs to the steroid hormone receptor superfamily, including estrogen, progesterone, glucocorticoid, androgen, and thyroid hormone receptors.
Vitamin D modulates calcium and phosphorus homeostasis, bone remodeling, cell growth regulation, and differentiation.
VDR is found in the intestine, bone, kidney, epidermis, and endocrine immune cells.
VDR binding within the IL-2 enhancer represses T-cell proliferation and IL-2 gene expression.
Formation of VDR/retinoic X receptor (RXR) heterodimers in the presence of intracellular 1,25(OH)₂D₃ interferes with NFATp/Fos/Jun/DNA complex assembly and subsequent IL-2 transcription.
The corresponding gene for the vitamin D receptor is NR1I1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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