Thermo Fisher Scientific STIM1 Polyclonal Antibody
Human STIM1 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ELISA, IP 등 다양한 응용에 적합. 보존제가 없는 액상 형태이며, Protein A 및 항원 친화 크로마토그래피로 정제됨. STIM1/Orai1 칼슘 신호전달 연구에 유용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:2,000–1:10,000 |
| ELISA | 1:25,000–1:50,000 |
| Immunoprecipitation (IP) | 0.2–1 µL/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human STIM1 protein (Met1–Asp213) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2788298 |
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
This antibody has specificity for Human STIM1/GOK.
Target Information
STIM1 (stromal interacting molecule; also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Following a decrease in luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm.
STIM1 also associates with growing ends of microtubules and is involved in endoplasmic reticulum tubule extension.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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