Thermo Fisher Scientific Phospho-EGFR (Tyr1148) Polyclonal Antibody
EGFR Tyr1148 인산화 부위를 특이적으로 인식하는 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF에 사용 가능. 인간 및 설치류 반응성. 항원 친화 크로마토그래피로 정제된 액상 항체로, 보존제 없이 -20°C에서 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding pTyr1148 of human EGF receptor |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10982211 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with other tyrosine-phosphorylated ErbB family members.
Target Information
EGFR (epidermal growth factor receptor) is a receptor tyrosine kinase that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer, and EGFR is a target for both biological and small-molecule therapeutics.
The EGFR gene is located on chromosome 7 in humans and belongs to the HER/ErbB family, which includes ERbB2, ERbB3, and ERbB4.
EGFR is a transmembrane receptor; binding of ligands such as EGF and TGF-α to its extracellular domain induces dimerization and autophosphorylation of tyrosine residues in the cytoplasmic domain.
Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, and squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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