Thermo Fisher Scientific CD16/CD32 Monoclonal Antibody (FCR4G8)

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1:25-1:50

-

Functional Assay (Functional)

Assay-dependent

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

FCR4G8

Immunogen

PU5 1.8 IOE7 Balb/c mouse cell line. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD16/CD32,uniProtId:P08101-1,ncbiNodeId:10090,antigenRange:1-330,antigenLength:330,antigenImageFileName:MA5-16680_CD16CD32_P08101-1_Rat.svg,antigenImageFileNamePDP:MA5-16680_CD16CD32_P08101-1_Rat_PDP.jpeg,sortOrder:1},{targetFamily:CD16/CD32,uniProtId:P08508-1,ncbiNodeId:10090,antigenRange:1-261,antigenLength:261,antigenImageFileName:MA5-16680_CD16CD32_P08508-1_Rat.svg,antigenImageFileNamePDP:MA5-16680_CD16CD32_P08508-1_Rat_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538174

Product Specific Information

This product is a low-endotoxin, preservative-free formulation. Endotoxin level is <0.01EU/ug.

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Target Information

CD16 is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by natural killer (NK) cells, and some T cells and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC (antibody dependent cell mediated cytotoxicity) by NK cells. IN comparison, CD32 is a 40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII), and mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Further, CD32 is involved in the phagocytosis of immune complexes, the regulation of antibody production by B-cells, and variations of the CD32 gene may increase vulnerability to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms of CD32 have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.