Thermo Fisher Scientific Connexin 43 Monoclonal Antibody (M496)
Connexin 43 단백질을 인식하는 Mouse monoclonal 항체로, Western blot 및 면역형광(ICC/IF)에 적합합니다. 인간, 생쥐, 랫트 시료에서 반응하며, gap junction 연구에 유용합니다. 항원 친화 크로마토그래피로 정제되었으며, -20°C에서 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M496 |
| Immunogen | Clone M496 was generated from a peptide fragment in the C-terminal region of human connexin-43. This sequence is highly conserved in mouse and rat connexin-43. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942691 |
Product Specific Information
This antibody detects a 43 kDa protein corresponding to connexin-43 on SDS-PAGE immunoblots of mouse brain. It is also effective for immunofluorescent labeling of gap junctions in cells.
Target Information
Connexin 43 (Cx43) is a member of the gap junction protein family. Connexins assemble as hexamers and are transported to the plasma membrane to form hemichannels that connect adjacent cells, creating gap junctions. These structures enable intercellular communication critical for development and cell growth regulation.
Phosphorylation of Cx43 regulates gap junction assembly and function. Protein kinase C (PKC) phosphorylates Ser368 of Cx43, reducing cell-to-cell communication. Src kinase can also phosphorylate Cx43, influencing gap junction signaling.
Connexin 43 is the major gap junction protein in the heart, essential for synchronized cardiac contraction and embryonic development. It is targeted by multiple kinases that regulate myocardial cell-cell coupling. A related pseudogene, GJA1P, is located on chromosome 5. Mutations in connexin-related genes are associated with disorders such as Charcot-Marie-Tooth disease, oculodentodigital dysplasia, and heart malformations.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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