Thermo Fisher Scientific Connexin 43 Monoclonal Antibody (M496)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

This antibody detects a 43 kDa protein corresponding to connexin-43 on SDS-PAGE immunoblots of mouse brain. It is also effective for immunofluorescent labeling of gap junctions in cells.

Target Information

Connexin 43 (Cx43) is a member of the gap junction protein family. Connexins assemble as hexamers and are transported to the plasma membrane to form hemichannels that connect adjacent cells, creating gap junctions. These structures enable intercellular communication critical for development and cell growth regulation.

Phosphorylation of Cx43 regulates gap junction assembly and function. Protein kinase C (PKC) phosphorylates Ser368 of Cx43, reducing cell-to-cell communication. Src kinase can also phosphorylate Cx43, influencing gap junction signaling.

Connexin 43 is the major gap junction protein in the heart, essential for synchronized cardiac contraction and embryonic development. It is targeted by multiple kinases that regulate myocardial cell-cell coupling. A related pseudogene, GJA1P, is located on chromosome 5. Mutations in connexin-related genes are associated with disorders such as Charcot-Marie-Tooth disease, oculodentodigital dysplasia, and heart malformations.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.