Thermo Fisher Scientific AKT1/AKT2 Recombinant Rabbit Monoclonal Antibody (1G0K5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

1G0K5

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 381-480 of human AKT1/2 (P31749). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:AKT1/AKT2,uniProtId:P31749-1,ncbiNodeId:9606,antigenRange:381-480,antigenLength:480,antigenImageFileName:MA5-42793_AKT1AKT2_P31749-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42793_AKT1AKT2_P31749-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:AKT1/AKT2,uniProtId:P31751-1,ncbiNodeId:9606,antigenRange:381-480,antigenLength:481,antigenImageFileName:MA5-42793_AKT1AKT2_P31751-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42793_AKT1AKT2_P31751-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.72 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2911934

Product Specific Information

Positive test controls include: PC-12, PC-3, C2C12.

Immunogen sequence: SGLLKKDPKQ RLGGGSEDAK EIMQHRFFAG IVWQHVYEKK LSPPFKPQVT SETDTRYFDE EFTAQMITIT PPDQDDSMEC VDSERRPHFP QFSYSASGTA

Target Information

AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at Ser-50 negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insulin-stimulated glucose transport. AKT regulates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at Ser-21 and GSK3B at Ser-9, resulting in inhibition of its kinase activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.