Thermo Fisher Scientific Cyclin A1/A2 Recombinant Rabbit Monoclonal Antibody (10O1C8)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 400 µg-600 µg extracts of

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

10O1C8

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 250-465 of human Cyclin A1/A2 (P20248). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cyclin A,uniProtId:P78396-1,ncbiNodeId:9606,antigenRange:250-465,antigenLength:465,antigenImageFileName:MA5-42789_Cyclin_A_P78396-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42789_Cyclin_A_P78396-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2911930

Product Specific Information

Positive test controls include: HCT116, Jurkat, Mouse thymus.

Immunogen sequence: RGKLQLVGTA AMLLASKFEE IYPPEVAEFV YITDDTYTKK QVLRMEHLVL KVLTFDLAAP TVNQFLTQYF LHQQPANCKV ESLAMFLGEL SLIDADPYLK YLPSVIAGAA FHLALYTVTG QSWPESLIRK TGYTLESLKP CLMDLHQTYL KAPQHAQQSI REKYKNSKYH GVSLLNPPET LNL

Target Information

The critical role that the family of regulatory proteins known as cyclins play in eukaryotic cell cycle regulation is well established. The best-characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of maturing promoting factor. Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin-dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DRTF1 and E2F and retinoblastoma protein, p110. A second form of cyclin A, named cyclin A1 because of its high sequence homology to Xenopus cyclin A1, is most highly expressed in germ cells. It has been proposed that cyclin A1 can associate with Cdk2, p39 and Cdc2 p34.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.