Thermo Fisher Scientific Mouse TNF-alpha Recombinant Protein

Applications

• Western Blot (WB): Assay-dependent
• ELISA (ELISA): Assay-dependent
• Immunoprecipitation (IP): Assay-dependent
• Control (Ctrl): Assay-dependent
• Miscellaneous PubMed (Misc): View 4 publications

Product Specifications

Product Specific Information

The mature, soluble form of mouse TNF-alpha was expressed in Pichia pastoris (strain KM71H).
GenBank Accession # P06804, amino acids 80–235.
Protein verification was performed by N-terminal sequencing, beginning with the following 10 amino acids: LRSSSQNSSD.
Purity is >95% by SDS-PAGE analysis.

Activity:
The biological activity of recombinant mouse TNF-alpha was measured by its cytolytic effect on mouse WEHI-13VAR cells in the presence of Actinomycin D.

Use:
For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments.

Reconstitution and Dilution:

• Store lyophilized material at 4°C.
• Reconstitute in 0.5 mL of distilled or deionized water.
• Dilute in a buffer containing a carrier protein, aliquot, and store at or below –70°C.
• Avoid multiple freeze-thaw cycles.
• PBS with 1% BSA or a neutral medium containing <10% FCS are suitable buffers.

Target Information

TNF-alpha is a multifunctional proinflammatory cytokine belonging to the tumor necrosis factor (TNF) superfamily. It is mainly secreted by macrophages and binds to receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR.
This cytokine regulates immune cell activity, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.
TNF-alpha typically exists as multimers of two, three, or five noncovalently linked units but appears as a single 17 kDa band under non-reducing SDS-PAGE conditions.
Knockout studies in mice indicate a neuroprotective function, and TNF-alpha induces tumor necrosis when injected into tumor-bearing mice.
It also plays roles in immune responses to bacterial, viral, parasitic, and fungal infections, and contributes to the necrosis of specific tumors.
TNF-alpha causes cytolysis or cytostasis of certain transformed cells and acts synergistically with interferon-gamma in cytotoxicity.
This cytokine is implicated in autoimmune diseases, insulin resistance, and cancer.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.