Thermo Fisher Scientific Mouse IL-4 Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Control (Ctrl)

Assay-dependent

-

Product Specifications

Species

Mouse

Expression System

P. pastoris

Amino acid sequence

Mouse IL-4, amino acids 21-140 (Accession # P07750)

Molecular weight

13.6 kDa

Class

Recombinant

Type

Protein

Purity

>95% by SDS-PAGE

Activity

ED50 = 3.3-4.9 ng/mL: Determined by the proliferation of mouse HT-2 cells.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

PBS

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

Mature mouse IL-4 was expressed in Pichia pastoris, X-33. GenBank Accession # P07750, amino acids 21 - 140. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: HIHGCDKNHL. Purity is >95% by SDS-PAGE analyses.

Activity: The biological activity of recombinant mouse IL-4 was determined by measuring the proliferation of mouse HT-2 cells.

Use: For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments.

Reconstitution and Dilution: Store lyophilized material at 4ºC. Reconstitute in 0.5 mL of distilled or deionized water. Dilute in a buffer-containing carrier protein, aliquot, and store at or below -70ºC, avoiding multiple freeze-thaw cycles. PBS with 1% BSA or a neutral media containing <10% FCS are suitable buffers.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.