Thermo Fisher Scientific Mouse IL-4 Recombinant Protein

Applications

• Western Blot (WB): Assay-dependent
• ELISA: Assay-dependent
• Immunoprecipitation (IP): Assay-dependent
• Control (Ctrl): Assay-dependent

Product Specifications

Product Specific Information

Mature mouse IL-4 was expressed in Pichia pastoris X-33 (GenBank Accession # P07750, amino acids 21–140).
The expressed protein was N-terminal sequenced and begins with the following 10 amino acids: HIHGCDKNHL.
Purity is >95% by SDS-PAGE analyses.

Activity: The biological activity of recombinant mouse IL-4 was determined by measuring the proliferation of mouse HT-2 cells.

Use: For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments.

Reconstitution and Dilution:

• Store lyophilized material at 4ºC.
• Reconstitute in 0.5 mL of distilled or deionized water.
• Dilute in a buffer containing carrier protein, aliquot, and store at or below -70ºC.
• Avoid multiple freeze-thaw cycles.
• Suitable buffers: PBS with 1% BSA or neutral media containing <10% FCS.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells and serves as a ligand for the interleukin 4 receptor.
IL-4 has a broad range of actions and also binds IL-13, contributing to overlapping functions.
STAT6 plays an important role in mediating IL-4 immune regulatory signaling.
IL-4, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q, with IL-4 located near IL-13.
These genes are coordinated by long-range regulatory elements over a 120 kb region.
IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.