Thermo Fisher Scientific ETS1 Polyclonal Antibody
ETS1 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 IHC(Paraffin)에서 검증됨. Human, Mouse, Rat에 반응하며, 항원 친화 크로마토그래피로 정제됨. 고순도(>95%)이며, 장기 보관 시 -20°C에서 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the N-terminus of Human ETS1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2802880 |
Product Specific Information
Purity is >95% by SDS-PAGE.
Target Information
ETS1 (c-Ets-1; v-ets erythroblastosis virus E26 oncogene homolog 1 (avian); ETS-1; EWSR2; p54) is a 441 amino acid protein and a member of the DNA-binding ETS protein family.
It is a multifunctional protein consisting of:
- N-terminal pointed domain
- Central transactivation domain
- Two auto-inhibitory domains flanking a conserved C-terminal ETS domain
ETS1 interacts with multiple factors such as PEBP2alphaA, p300/CBP, and mediates transactivation of Osteopontin, Presenilin-1, Stromelysin, and Collagenase.
It is activated by Ras/MAPK signaling and upregulated in resting T-cells.
Its activity is modulated by AP-1, Sp-1, c-myb, MafB, and others.
Sp100 activates ETS1 in nuclear bodies.
ETS1 interacts with EAP1/Daxx to repress MMP1 and BCL2 transactivation, and with STAT6 to modulate Socs-1 expression induced by IL-4.
It also interacts with GATA3 to positively regulate Tax-1 dependent IL-5 expression.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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