Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (G8.8), eFluor 450, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant Violet 711
• Brilliant Violet 786
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

Description:
The G8.8 monoclonal antibody reacts with the 40 kDa mouse EpCAM (epithelial cellular adhesion molecule), also known as EGP40, 17-1A antigen, TACSTD1, and CD326. The immunogen used was the TE-71 thymic epithelial cell line. CD326 is expressed on the majority of epithelial cells and is considered a pan-carcinoma antigen. It mediates calcium-independent, homophilic cell-cell adhesion and may function as a growth factor receptor. The antigen is used as a target in immunotherapy for human carcinomas. CD326 binds LAIR-1 (CD305) and LAIR-2 (CD306) to inhibit cellular activation and inflammation, playing an important role in tumor biology.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of TE-71 cell line. Recommended ≤1 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Optimize concentration per assay.

Fluorochrome Information:
eFluor® 450 is an alternative to Pacific Blue®, excited at 405 nm and emits at 445 nm. Ensure instrument compatibility.

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

Ep-CAM (epithelial adhesion molecule, ESA) is a ~40 kDa transmembrane glycoprotein expressed in epithelial cells. It functions as a homotypic, calcium-independent adhesion molecule and influences cell cycle and proliferation by inducing proto-oncogene c-myc and cyclins A/E. Ep-CAM-mediated adhesions negatively regulate cadherin-based adhesions, affecting epithelial differentiation and growth. Overexpression correlates with enhanced epithelial proliferation and is a marker for epithelial-derived tumors. Expressed on baso-lateral surfaces of most simple epithelia and carcinoma types, Ep-CAM helps distinguish adenocarcinomas from pleural mesotheliomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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