Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (G8.8), eFluor 450, eBioscience
마우스 EpCAM(CD326)을 인식하는 G8.8 클론 단일클론항체로, eFluor 450 형광표지. Flow cytometry에 최적화되어 있으며 다양한 형광 형식으로 제공. 405 nm 레이저로 여기, 445 nm에서 방출. 암세포 표지 및 세포 접착 연구용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 13 |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 1 |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 1 |
| Immunocytochemistry (ICC/IF) | - | 11 |
| Flow Cytometry (Flow) | 1 µg/test | 72 |
| Affinity Purification (AP) | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | G8.8 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10717391 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant Violet 711
- Brilliant Violet 786
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
Description:
The G8.8 monoclonal antibody reacts with the 40 kDa mouse EpCAM (epithelial cellular adhesion molecule), also known as EGP40, 17-1A antigen, TACSTD1, and CD326. The immunogen used was the TE-71 thymic epithelial cell line. CD326 is expressed on the majority of epithelial cells and is considered a pan-carcinoma antigen. It mediates calcium-independent, homophilic cell-cell adhesion and may function as a growth factor receptor. The antigen is used as a target in immunotherapy for human carcinomas. CD326 binds LAIR-1 (CD305) and LAIR-2 (CD306) to inhibit cellular activation and inflammation, playing an important role in tumor biology.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of TE-71 cell line. Recommended ≤1 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Optimize concentration per assay.
Fluorochrome Information:
eFluor® 450 is an alternative to Pacific Blue®, excited at 405 nm and emits at 445 nm. Ensure instrument compatibility.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
Ep-CAM (epithelial adhesion molecule, ESA) is a ~40 kDa transmembrane glycoprotein expressed in epithelial cells. It functions as a homotypic, calcium-independent adhesion molecule and influences cell cycle and proliferation by inducing proto-oncogene c-myc and cyclins A/E. Ep-CAM-mediated adhesions negatively regulate cadherin-based adhesions, affecting epithelial differentiation and growth. Overexpression correlates with enhanced epithelial proliferation and is a marker for epithelial-derived tumors. Expressed on baso-lateral surfaces of most simple epithelia and carcinoma types, Ep-CAM helps distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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