Thermo Fisher Scientific PRKAR1B Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 1 publication 1 publication

ELISA (ELISA)

1 µg/mL

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Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 190-290 of human PRKAR1B (NP_0027261) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PRKAR1B,uniProtId:P31321-1,ncbiNodeId:9606,antigenRange:190-290,antigenLength:381,antigenImageFileName:PA5-87652_PRKAR1B_P31321-1_Rabbit.svg,antigenImageFileNamePDP:PA5-87652_PRKAR1B_P31321-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.3 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2804321

Product Specific Information

Immunogen sequence: WVTNISEGGS FGELALIYGT PRAATVKAKT DLKLWGIDRD SYRRILMGST LRKRKMYEEF LSKVSILESL EKWERLTVAD ALEPVQFEDG EKIVVQGEPG D; Positive Samples: U-251MG, HeLa, Jurkat, HepG2, BT-474, Mouse brain, Mouse lung, Rat brain, Rat testis; Cellular Location: Cell membrane

Target Information

The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified that each represent specific gene products. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcrip- tion factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.