Thermo Fisher Scientific MAPK1 Monoclonal Antibody (OTI1C11), TrueMAB
인간 MAPK1(ERK2)을 인식하는 Mouse IgG1 단일클론 항체로, Western blot 및 Immunoprecipitation에 최적화됨. 동결건조 형태이며, PBS 및 trehalose 버퍼로 안정화. 연구용으로 세포 신호전달 및 MAPK 경로 연구에 적합.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Immunoprecipitation (IP)
- Tested Dilution: 2–4 µg/mg
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1C11 |
| Immunogen | Full length human recombinant protein of human MAPK1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
MAPK1 (ERK2) is a serine/threonine kinase that activates p90 RSK, MSK, ELK1, and Stat3, playing a key role in the MAPK signaling pathway.
MAP kinases serve as integration points for multiple biochemical signals and regulate diverse cellular processes including proliferation, differentiation, transcription regulation, and development.
Activation requires phosphorylation by upstream kinases, followed by nuclear translocation to phosphorylate nuclear targets.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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