Thermo Fisher Scientific E2-25K Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000–1:5,000
• Publications: [참조 없음]

Immunoprecipitation (IP)

• Tested Dilution: 2–10 µg/mg lysate
• Publications: [참조 없음]

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Based on 100% sequence identity, this antibody is predicted to react with Bovine.

Target Information

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, with or without the BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of Lys-48-linked polyubiquitin chains.
Does not transfer ubiquitin directly but elongates monoubiquitinated substrate proteins. Mediates degradation of short-lived and abnormal proteins, including ER-associated degradation (ERAD) of misfolded lumenal proteins.
Ubiquitinates huntingtin and may mediate foam cell formation by suppressing apoptosis of lipid-bearing macrophages via ubiquitination and degradation of p53/TP53.
Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; supports ubiquitination of NFKB1 in vitro.
During cytomegalovirus infection, may participate in US11-dependent degradation of MHC class I heavy chains after export from the ER to the cytosol.
In viral infections, may be involved in HPV E7 protein-dependent degradation of RB1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.