Thermo Fisher Scientific E2-25K Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000–1:5,000

Immunoprecipitation (IP)

• Tested Dilution: 2–10 µg/mg lysate

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Based on 100% sequence identity, this antibody is predicted to react with Bovine

Target Information

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of Lys-48-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate proteins. Mediates selective degradation of short-lived and abnormal proteins, including ER-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin and may mediate foam cell formation by suppressing apoptosis of lipid-bearing macrophages through ubiquitination and degradation of p53/TP53. Proposed involvement in ubiquitination and proteolytic processing of NF-kappa-B; supports ubiquitination of NFKB1 in vitro. May play a role in viral infection responses, including cytomegalovirus US11-dependent degradation of MHC class I heavy chains and HPV E7-dependent degradation of RB1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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