Thermo Fisher Scientific Phospho-MEK1/MEK2 (Ser217, Ser221) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser218/222 of human MAP2K1/2 conjugated to KLH if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MEK1/2,uniProtId:Q02750-1,ncbiNodeId:9606,antigenRange:218|221|222,antigenLength:393,antigenImageFileName:PA1-4606_MEK12_Q02750-1_Rabbit.svg,antigenImageFileNamePDP:PA1-4606_MEK12_Q02750-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:MEK1/2,uniProtId:P36507-1,ncbiNodeId:9606,antigenRange:218|221|222,antigenLength:400,antigenImageFileName:PA1-4606_MEK12_P36507-1_Rabbit.svg,antigenImageFileNamePDP:PA1-4606_MEK12_P36507-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_560046

Product Specific Information

This antibody is predicted to react with bovine, canine, chicken, non-human primate, rat, Xenopus and zebrafish based on 100% sequence homology.

In Western blot, this antibody detects an ~45 kDa protein representing the Phospho-MEK1/2 (Ser218, 222) protein.

Target Information

MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). In brain, this pathway also plays a key role in synaptic (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.