Thermo Fisher Scientific AMIGO1 Monoclonal Antibody (L86/33), FITC
AMIGO1 단백질을 인식하는 FITC 결합 단클론 항체로, 인간, 마우스, 랫트 시료에 반응합니다. Western blot, IHC, ICC/IF에 사용 가능하며, 단백질 G로 정제된 액상 형태입니다. 신경세포 성장 및 축삭 형성 연구에 적합합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunohistochemistry (IHC) | Assay-dependent | |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | L86/33 |
| Immunogen | Fusion protein of amino acids 394–492 (cytoplasmic C-terminus) of mouse AMIGO-1 |
| Conjugate | FITC |
| Excitation/Emission Max | 498/517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage conditions | 4°C, store in dark |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932074 |
Additional Formats
- Unconjugated (MA5-27611)
- APC (MA5-45619)
- PE (MA5-45622)
- PerCP (MA5-45621)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45620 was sufficient for detection of AMIGO-1 in 20 µg of rat brain membrane lysate by colorimetric immunoblot using goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 60–80 kDa depending on maturity/glycosylation.
This antibody was formerly sold as clone S86-36.
Target Information
AMIGO1 promotes growth and fasciculation of neurites from cultured hippocampal neurons. It may be involved in fasciculation and myelination of developing neural axons, and play a role in regeneration and neural plasticity in the adult nervous system. It mediates cell-cell interactions and contributes to signal transduction through its intracellular domain. When assembled with KCNB1, it modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1.
[UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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