Thermo Fisher Scientific AMIGO1 Monoclonal Antibody (L86/33), APC
AMIGO1 단백질을 인식하는 마우스 단클론 항체로, APC 형광 표지. Western blot, IHC, ICC/IF에 사용 가능. 인간, 마우스, 랫트 반응성. 단백질 G 정제, 1 mg/mL 농도. 4°C 보관, 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | L86/33 |
| Immunogen | Fusion protein (amino acids 394–492, cytoplasmic C-terminus of mouse AMIGO-1) |
| Conjugate | APC (Allophycocyanin) |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932073 |
Additional Formats
- Unconjugated (MA5-27611)
- FITC (MA5-45620)
- PE (MA5-45622)
- PerCP (MA5-45621)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45619 was sufficient for detection of AMIGO-1 in 20 µg of rat brain membrane lysate using colorimetric immunoblot analysis with goat anti-mouse IgG:HRP as secondary antibody.
Detects approximately 60–80 kDa depending on maturity/glycosylation.
This antibody was formerly sold as clone S86-36.
Target Information
AMIGO1 promotes growth and fasciculation of neurites from cultured hippocampal neurons. It may be involved in fasciculation and myelination of developing neural axons and play a role in regeneration and neural plasticity in the adult nervous system. AMIGO1 mediates homophilic and heterophilic cell-cell interactions and contributes to signal transduction through its intracellular domain. When assembled with KCNB1, it modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1.
(Source: UniProt)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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