Thermo Fisher Scientific ERK1 Polyclonal Antibody
ERK1 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC, IP 등 다양한 응용에 사용 가능. Human, Mouse, Rat에 반응하며, 항원 친화 크로마토그래피로 정제됨. 세포 내 핵 위치 확인에 유용하며, 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
| Immunocytochemistry (ICC/IF) | 1:200 |
| Immunoprecipitation (IP) | 2–5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the internal segment of human ERK1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Mouse brain, heart, kidney, and spleen lysates
- Cellular location: Nuclear
Target Information
MAPK3 (ERK1) is a serine/threonine kinase that acts as an important activator of p90, RSK, MSK, ELK1, and Stat3, and participates in the MAPK pathway.
ERK1 is widely expressed and regulates meiosis, mitosis, and post-mitotic functions in differentiated cells.
It is activated by various stimuli such as growth factors, cytokines, viral infection, and G protein-coupled receptor ligands.
Functionally, ERK1 is involved in signaling cascades that regulate proliferation, differentiation, and cell cycle progression in response to extracellular signals.
Upon activation, ERK1 translocates to the nucleus where it phosphorylates nuclear targets.
Alternatively spliced transcript variants encoding different protein isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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