Thermo Fisher Scientific ERK1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

2-10 µg/mL

-

Immunocytochemistry (ICC/IF)

1:200

-

Immunoprecipitation (IP)

2-5 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corrsponding to the internal segment of human ERK1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with proprietary stabilizer

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Positive control: Mouse brain, heart, kidney and spleen lysates

Cellular location: Nuclear.

Target Information

MAPK3 (ERK1) is a serine/threonine kinase that is an important activator of p90, RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. ERK1 is widely expressed and involved in the regulation of meiosis, mitosis, and post-mitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. Functionally, ERK1 is involved with a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. ERK1 is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms of ERK1 have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.