Thermo Fisher Scientific P-Selectin Monoclonal Antibody (AK-6), FITC

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Rhesus monkey

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

AK-6

Immunogen

Human platelet membrane glycoproteins if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:P-Selectin (CD62P),uniProtId:P16109-1,ncbiNodeId:9606,antigenRange:1-830,antigenLength:830,antigenImageFileName:MA5-16568_P-Selectin_CD62P_P16109-1_House_mouse.svg,antigenImageFileNamePDP:MA5-16568_P-Selectin_CD62P_P16109-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

FITC FITC FITC

View additional formats

• Unconjugated
• PE
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1% BSA

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538072

Product Specific Information

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Mouse anti Human CD62P antibody, clone AK-6 recognizes the CD62P, also known as P-selectin, Granule membrane protein 140, GMP140, Leukocyte-endothelial cell adhesion molecule 3 or Platelet activation dependent granule-external membrane protein.

Target Information

P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type 1 transmembrane glycoprotein, and is one of the most commonly studied proteins that regulate cell rolling. P-selectin is stored in the Weibel-Palade bodies of endothelial cells, as well as in a-granules of platelets. From there, it can be rapidly brought to the cell surface after exposure to thrombin, histamine, complement 5a, Ca21 ionophores, or adenosine diphosphate. P-selectin protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. P-selectin plays an important role in adhesive processes between leucocytes and endothelial cells, and is a calcium dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens found on neutrophils and monocytes. P-selectin is constitutively expressed in inflammation and contributes to atherogenesis, thrombosis and tissue destruction. Clinically, P-selectin is used to distinguish heparin induced thrombocytopenia with and without thrombosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.