Thermo Fisher Scientific P-Selectin Monoclonal Antibody (AK-6)

Applications and Tested Dilution

Product Specifications

Additional Formats

• FITC
• PE
• Request custom conjugation

Product Specific Information

• For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
• A suggested positive control for immunohistochemical applications is human tonsil.
• Mouse anti-Human CD62P antibody, clone AK-6 recognizes CD62P (P-selectin), also known as Granule membrane protein 140 (GMP140), Leukocyte-endothelial cell adhesion molecule 3 (LECAM3), or Platelet activation-dependent granule-external membrane protein.

Target Information

P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type I transmembrane glycoprotein that regulates cell rolling. It is stored in the Weibel-Palade bodies of endothelial cells and α-granules of platelets and is rapidly translocated to the cell surface upon activation by thrombin, histamine, complement 5a, Ca²⁺ ionophores, or ADP.
P-selectin mediates interactions between activated endothelial cells or platelets and leukocytes, playing a key role in leukocyte-endothelial adhesion. It binds to sialylated Lewis carbohydrate antigens on neutrophils and monocytes in a calcium-dependent manner.
P-selectin expression is associated with inflammation, atherogenesis, thrombosis, and tissue injury. Clinically, it aids in distinguishing heparin-induced thrombocytopenia with or without thrombosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.