Thermo Fisher Scientific PFKP Polyclonal Antibody
PFKP 단백질을 인식하는 Rabbit Polyclonal 항체로, Human, Mouse, Rat 시료에 반응합니다. Western blot, IHC, ICC/IF, ELISA에 사용 가능하며, 친화 크로마토그래피로 정제되었습니다. PBS/glycerol buffer에 보관되며, 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 336–540 of human PFKP (NP_002618.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.41 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914726 |
Product Specific Information
Positive test controls include: MCF7, Jurkat, Mouse brain, Mouse heart, Rat testis, Rat brain.
The target is typically localized in the cytoplasm.
Immunogen sequence:
ATPDTPACVV SLNGNHAVRL PLMECVQMTQ DVQKAMDERR FQDAVRLRGR SFAGNLNTYK RLAIKLPDDQ IPKTNCNVAV INVGAPAAGM NAAVRSAVRV GIADGHRMLA IYDGFDGFAK GQIKEIGWTD VGGWTGQGGS ILGTKRVLPG KYLEEIATQM RTHSINALLI IGGFEAYLGL LELSAAREKH EEFCVPMVMV PATVS
Target Information
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the γ phosphate of ATP, onto an acceptor amino acid in a substrate protein. Through this mechanism, protein kinases mediate most signal transduction in eukaryotic cells, regulating metabolism, transcription, cell cycle progression, cytoskeletal rearrangement, apoptosis, and differentiation.
The protein kinase family includes more than 500 gene products and is classified into 8 major groups based on catalytic domain sequence similarity (tyrosine or serine/threonine kinases).
The AGC kinase group includes 63 kinases such as PKA, PKG, PKC, RAC/Akt, ARK, and RSK families.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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