Thermo Fisher Scientific beta Galactosidase Fusion Protein Polyclonal Antibody, eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

1:400

View 1 publication 1 publication

Immunohistochemistry (IHC)

Assay-Dependent

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunohistochemistry - Free Floating (IHC (Free))

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bacteria

Published species

Bacteria, Fruit fly, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with BSA, 0.02% gelatin

Contains

0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468331

Product Specific Information

Description: The rabbit polyclonal antibody reacts with bBeta galactosidase. Beta-galactosidase is a large 464 kDa tetramer when assayed by western blot under nonreducing conditions and 116 kDa monomer under reducing conditions. The beta-galactosidase activity allows for the quick determination of cells expressing the lacZ gene. The lacZ gene is used as a reporter construct for determination of transfection efficiency as well as histochemical localization following transfection of eukaryotic cells. The lacZ gene product beta-galactosidase catalyzes the hydrolysis of the substrate 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal) to produce a blue color that is easily visualized with a microscope.

Applications Reported: Purified Anti-beta galactosidase fusion proteins poly has been reported for use in immunoblotting (WB), and immunohistochemical staining.

Applications Tested: Purified Anti-beta galactosidase fusion proteins poly has been tested by immunoblotting (WB). (1:400 starting dilution). It is recommended that this antibody be titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Beta Galactosidase encodes a member of the glycosyl hydrolase 35 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme. This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. Mutations in this gene have been associated with GM1-gangliosidosis and Morquio B syndrome. Diseases associated with GLB1 include Gm1-Gangliosidosis, Type I and Gm1-Gangliosidosis, Type Ii.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.