Thermo Fisher Scientific beta Galactosidase Fusion Protein Polyclonal Antibody, eBioscience
베타 갈락토시다아제 융합 단백질을 인식하는 토끼 폴리클로날 항체로, WB 및 IHC에 사용 가능. Affinity chromatography로 정제되었으며 0.2 mg/mL 농도의 액상 형태. 세포 내 lacZ 발현 검출 및 트랜스펙션 효율 평가에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:400 | View 1 publication |
| Immunohistochemistry (IHC) | Assay-Dependent | View 3 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | – | View 1 publication |
| Immunohistochemistry - Free Floating (IHC (Free)) | – | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bacteria |
| Published Species | Bacteria, Fruit fly, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with BSA, 0.02% gelatin |
| Contains | 0.1% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_468331 |
Product Specific Information
The rabbit polyclonal antibody reacts with beta-galactosidase. Beta-galactosidase is a large 464 kDa tetramer when assayed by western blot under nonreducing conditions and a 116 kDa monomer under reducing conditions.
The beta-galactosidase activity allows for quick determination of cells expressing the lacZ gene. The lacZ gene is used as a reporter construct for transfection efficiency determination and histochemical localization in eukaryotic cells.
The lacZ gene product catalyzes the hydrolysis of the substrate 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal), producing a blue color easily visualized under a microscope.
Applications Reported: Purified Anti-beta galactosidase fusion proteins poly has been reported for use in immunoblotting (WB) and immunohistochemical staining.
Applications Tested: Tested by immunoblotting (WB) at a 1:400 starting dilution. It is recommended to titrate for optimal performance.
Purity: Greater than 90%, determined by SDS-PAGE.
Aggregation: Less than 10%, determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Beta Galactosidase encodes a member of the glycosyl hydrolase 35 family. Alternative splicing results in multiple transcript variants, including at least one that encodes a preproprotein processed to generate the mature lysosomal enzyme.
This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates.
Mutations in this gene are associated with GM1-gangliosidosis and Morquio B syndrome. Diseases associated with GLB1 include GM1-Gangliosidosis Type I and Type II.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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