Thermo Fisher Scientific Transferrin Receptor Monoclonal Antibody (MEM-75), Alexa Fluor 647

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

Flow Cytometry (Flow)

4 µLx100 µL whole blood or 10^6 cells

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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

MEM-75

Immunogen

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View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Alexa Fluor™ 647 Alexa Fluor™ 647 Alexa Fluor™ 647

View additional formats

• Unconjugated
• Alexa Fluor 700
• APC
• Biotin
• FITC
• PE
• Request custom conjugation

Excitation/Emission Max

650/671 nm View spectra

Form

Liquid

Purification

Size-exclusion chromatography

Storage buffer

PBS with 0.2% BSA

Contains

15mM sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539525

Product Specific Information

This antibody reacts with an extracellular epitope of CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and T lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes.

The antibody MEM-75 does not block binding of transferrin to the receptor.

Target Information

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.