Thermo Fisher Scientific CD8 alpha Monoclonal Antibody (53-6.7), Alexa Fluor 488
Alexa Fluor 488 형광으로 표지된 마우스 CD8α 단클론 항체(53-6.7). 유세포분석 및 면역형광에 최적화. Rat IgG2a 아이소타입, 0.5 mg/mL 농도. PBS + 15mM sodium azide 용액으로 4°C 암소 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | Assay-dependent | - |
| Flow Cytometry (Flow) | 2 µg/mL | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 53-6.7 |
| Immunogen | Mouse spleen cells |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Size-exclusion chromatography |
| Storage Buffer | PBS |
| Contains | 15mM sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2539527 |
Additional Available Formats
- Unconjugated
- APC
- FITC
- PE
- Custom conjugation available upon request
Product Specific Information
This antibody recognizes an extracellular epitope of murine CD8α (32–34 kDa; alpha chain of the CD8 antigen).
Target Information
Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family. It plays a key role in T cell signal transduction, differentiation, and activation. CD8 is mainly expressed on T cells as a heterodimer of CD8α and CD8β, functioning as a co-receptor for MHC class I molecules. CD8α is also found as a homodimer on subsets of T cells, NK cells, monocytes, and dendritic cells. Binding of MHC-I/peptide complexes to CD8 triggers recruitment of Lck kinase, promoting lymphokine production and cytotoxic T lymphocyte activation. Activated CTLs are crucial for pathogen and tumor cell clearance. Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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