Thermo Fisher Scientific E6AP Polyclonal Antibody
E6AP(UBE3A) 단백질을 인식하는 Rabbit Polyclonal 항체로, IHC(P) 및 ICC/IF에 사용 가능. Human, Mouse, Rat에 반응하며 항원 친화 정제 방식으로 제조됨. 액상 형태로 제공되며 4°C 또는 -20°C에서 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human UBE3A |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792270 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (91%), Rat (92%), Xenopus laevis (85%), Pig (94%), Rhesus Monkey (99%), Bovine (94%).
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
UBE3A (ubiquitin-protein ligase E3A, also known as E6AP) is an E3 ubiquitin-protein ligase that transfers ubiquitin from an E2 enzyme to its substrates.
Identified substrates include ARNTL/BMAL1, ARC, RAD23A, RAD23B, MCM7, annexin A1, PML tumor suppressor, and CDKN1B.
UBE3A may function as a cellular quality control ligase aiding in degradation of misfolded cytoplasmic proteins and plays a role in circadian clock regulation.
Defects in UBE3A are associated with Angelman syndrome (AS).
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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