Thermo Fisher Scientific ATF6 Polyclonal Antibody
ATF6 단백질을 인식하는 Thermo Fisher Scientific의 염소 유래 다클론 항체. Western blot, ICC/IF, Flow cytometry 등 다양한 응용에 적합. 인간 시료 반응성 확인. 액상 형태로 제공되며 -20°C에서 보관 권장. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.3–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | 10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide with sequence C-NSYRYDHSVSNDK |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2933693 |
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:128,000.
Target Information
ATF6 is a transcription factor that acts during endoplasmic reticulum (ER) stress by activating unfolded protein response target genes. It binds DNA on the 5'-CCAC[GA]-3' half of the ER stress response element (ERSE) and ERSE II sequences. Binding to ERSE requires NF-Y. ATF6 can form homodimers and heterodimers with ATF6 beta and interacts with NF-Y, GTF2I, YY1, and SRF. Under ER stress, the cleaved N-terminal domain translocates into the nucleus, functioning as a transcription factor. The cleavage is mediated sequentially by site-1 and site-2 proteases. ATF6 belongs to the bZIP family and is N-glycosylated and phosphorylated in vitro by MAPK14/P38MAPK.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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