Thermo Fisher Scientific CD8a Monoclonal Antibody (SK1), PerCP-eFluor 710, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (SK1), PerCP-eFluor 710, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 5 µL (0.125 µg)/test
  View 14 publications

Product Specifications

Additional Formats Available

• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 805
• Brilliant Violet 650
• eFluor 450
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PE-eFluor 610
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The SK1 monoclonal antibody reacts with human CD8a, a 32–34 kDa cell surface receptor expressed as CD8 alpha/beta heterodimers or CD8 alpha/alpha homodimers. CD8a is present on thymocytes, mature T cells, and NK cells, and binds to MHC class I molecules. Through association with p56lck kinase, CD8a contributes to T-cell development and activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and tested on normal human peripheral blood cells at 5 µL (0.125 µg)/test
• Recommended Filters: 710/50 bandpass (preferred), 695/40 bandpass (alternative)
• Laser: Blue Laser (488 nm excitation, 710 nm emission)
• Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein involved in T-cell signal transduction, differentiation, and activation. CD8 forms disulfide-linked heterodimers (CD8α/β) or homodimers (CD8α/α) and acts as a co-receptor for MHC class I molecules. It is expressed on T cells, NK cells, monocytes, and dendritic cells. Binding of MHC-I/peptide complexes triggers Lck kinase activation, leading to cytokine production and cytotoxic T lymphocyte (CTL) activation. CTLs play a key role in pathogen and tumor cell clearance, with differentiation enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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