Thermo Fisher Scientific PrP Monoclonal Antibody (EM-20)

Applications and Tested Dilution

• Western Blot (WB): 0.5 µg/mL
  Note: Non-reducing conditions are essential.

Product Specifications

Product Specific Information

This antibody recognizes human prion protein (PrP).
The diglycosylated form of PrP has an approximate molecular weight of 40 kDa, the monoglycosylated form is about 30 kDa, and the nonglycosylated form is around 19–21 kDa.
This antibody is suitable for distinguishing between the normal cellular prion protein (PrPc) and its conformationally changed form (PrPSc).

• Recommended Application: Western Blot (non-reducing conditions required)

Target Information

Human prion protein (PrP), also known as PRNP, is a ubiquitously expressed GPI-anchored cell surface glycoprotein that associates with lipid raft components and functions as a signaling molecule.
PrP is involved in apoptosis in a cell context-dependent manner, supports proliferation of epithelial cells, and influences the distribution of junction-associated proteins in human enterocytes.
Conversion of the normal cellular prion protein (PrPc) into its abnormal conformer (PrPSc) is the key event in the pathogenesis of prion neurodegenerative disorders such as Creutzfeldt-Jakob disease (CJD).
Whereas PrPc is rich in alpha-helices, the PrPSc form has a higher beta-sheet content and is resistant to proteinase K.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.