Thermo Fisher Scientific NFIB Monoclonal Antibody (1H2)
NFIB 단백질을 인식하는 Mouse monoclonal antibody (Clone 1H2). Western blot, IHC, ICC/IF, Flow cytometry에 적합. Human 시료에 반응하며, Protein G로 정제된 2 mg/mL 액상형 항체. -20°C에서 보관, 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1H2 |
| Immunogen | Recombinant protein within Human NFIB/NF1B2 aa 1–420 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2848808 |
Product Specific Information
Positive Control:
SH-SY-5Y, LO-2, rat heart tissue, human colon cancer tissue, human prostate tissue, human pancreas tissue, mouse liver tissue.
Target Information
NF-1 (also designated CTF) is a family of CCAAT box binding proteins that stimulate DNA replication and activate transcription. Human NF-1 mRNA reveals two forms arising from alternative splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA-binding domain and activates transcription through a proline-rich, carboxy-terminal transactivation domain. It recognizes and binds the adenovirus type 2 promoter and activates transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element is found in the upstream promoter regions of many eukaryotic genes, including Ha-Ras, alpha-globin, HSP70, GRP78, Histone H1, myelin basic protein, and Xenopus laevis vitellogenin gene promoters.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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