Thermo Fisher Scientific NFIB Monoclonal Antibody (1H5)
NFIB 단백질을 인식하는 Mouse monoclonal antibody(1H5)로, Western blot, IHC, Flow cytometry에 사용 가능. Human 시료에 반응하며, Protein G 정제 및 2 mg/mL 농도의 액상 형태. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1H5 |
| Immunogen | Recombinant protein within Human NFIB/NF1B2 aa 1–420 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2848809 |
Product Specific Information
Positive Control: SH-SY5Y, rat heart tissue, human pancreas tissue, mouse liver tissue.
Target Information
NF-1, also designated CTF, is a family of CCAAT box binding proteins that stimulate DNA replication and activate transcription. Human NF-1 mRNA reveals two forms arising from alternative splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA binding domain and activates transcription through a proline-rich, carboxy-terminal transactivation domain. It binds the adenovirus type 2 promoter and activates transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element is present in promoter regions of various eukaryotic genes such as Ha-Ras, alpha-globin, HSP70, GRP78, Histone H1, myelin basic protein, and Xenopus laevis vitellogenin gene promoter.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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