Thermo Fisher Scientific TIGIT Monoclonal Antibody (MBSA43), FITC, eBioscience

Applications

Immunohistochemistry (IHC)

• Not tested

Flow Cytometry (Flow)

• Tested dilution: 5 µL (0.125 µg)/test
• View 18 publications

Product Specifications

Additional Available Formats

• Alexa Fluor 488
• Alexa Fluor 647
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• eFluor 450
• Functional Grade
• NovaFluor Red 700
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The MBSA43 monoclonal antibody reacts with human T cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as VSTM3 or WUCAM. This 26 kDa protein belongs to the poliovirus receptor (PVR) family. TIGIT is expressed on NK cells, regulatory T cells, follicular T helper cells, memory CD4+ T cells, and CD8+ T cells, but not on B cells or naïve CD4+ T cells. Expression may be upregulated upon activation.

TIGIT interacts with members of the PVR and PVR-like families, including CD155, mediating interactions with antigen-presenting cells, fibroblasts, and endothelial cells expressing PVR-like proteins.

• Crossreactivity: Rhesus macaque
• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and validated for flow cytometry of normal human peripheral blood cells
• Recommended use: 5 µL (0.125 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)
• Excitation: 488 nm
• Emission: 520 nm
• Laser: Blue Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

TIGIT binds with high affinity to the poliovirus receptor (PVR), leading to increased IL10 secretion, decreased IL12B secretion, and suppression of T cell activation by promoting the generation of mature immunoregulatory dendritic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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